Barnase



Barnase (BA, 'BActerial RiboNucleASE') is a bacterial ribonuclease synthesized and secreted by the bacterium Bacillus amyloliquefaciens. The inhibitor barstar (BST) binds to it very tightly and prevents it from digestion of the cell’s RNA.

3D Structures of Barnase
3kch, 2f4y – BA cross-linked

2f56 - BA cross-linked + urea

2f5w - BA cross-linked + thiourea

2f5m - BA cross-linked + Bromo-ethanol

1fw7, 1bnr – BA - NMR

2kf3 – BA (mutant) – NMR

2kf4, 1b2x, 1a2p, 1bni, 1bnj – BA

1yvs – BA domain swapped

1b20, 1b21, 1b2z, 1bne, 1bnf, 1bng, 1brh, 1bri, 1brj, 1brk, 1bns, 1brg, 1bsa, 1bsb, 1bsc, 1bsd, 1bse, 1ban, 1bao – BA (mutant)

2kf5, 2kf6, 1brn, 1rnb – BA + polynucleotide

BA + BST
3da7 - BA (mutant) + BST

2za4, 1brs, 1bgs – BA + BST

1x1u, 1x1w, 1x1x, 1x1y, 1b27, 1b2u – BA + BST (mutant)

1b3s, 1b2s - BA (mutant) + BST (mutant)

2c4b – BA/MCOEETI (mutant)